STEP 4: EGG RETRIEVAL
All the instructions prior to the oocytes retrieval or egg pickup will be given on the day women receives trigger injection(usually GnRH agonist for final maturation of oocytes). The trigger injection is usually given late evening or in the night so as to schedule the pickup in morning around 34-36 hrs later. Aspiration of ovarian follicles under anaesthesia is done in OT adjacent to the IVF laboratory. This is done by trans-vaginal ultrasound technique. For this procedure a probe is inserted in to vagina and this project a picture into a monitor which enables the doctor to direct a needle into each follicle to retrieve the eggs. The fluid filled follicles are aspirated under slight suction in a test tube which is passed on to the embryologist.